Reconstitution of a chloroplast protein import channel
نویسندگان
چکیده
منابع مشابه
Reconstitution of a chloroplast protein import channel.
The chloroplastic outer envelope protein OEP75 with a molecular weight of 75 kDa probably forms the central pore of the protein import machinery of the outer chloroplastic membrane. Patch-clamp analysis shows that heterologously expressed, purified and reconstituted OEP75 constitutes a voltage-gated ion channel with a unit conductance of Lambda = 145pS. Activation of the OEP75 channel in vitro ...
متن کاملMechanisms of Chloroplast Protein Import in Plants
F. Kessler Laboratoire de Physiologie Végétale, Université de Neuchâtel, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland e-mail: [email protected] Abstract Most chloroplast proteins are encoded as preproteins by the nuclear genome. Their import into chloroplasts occurs post-translationally. An N-terminal pre-sequence, the transit peptide, contains the organellar targeting information. It ...
متن کاملThe mechanism of inactivation of a 50-pS envelope anion channel during chloroplast protein import.
The mechanism of import-competent precursor protein-induced inactivation of a 50-pS anion channel of the chloroplast envelope is investigated using single-channel recordings. The inactivation by precursor protein is the result of the induction of a long-lived closed state of the channel. The mean duration of this state does not depend on precursor concentration. From this it can be concluded th...
متن کاملMolecular chaperone involvement in chloroplast protein import.
Chloroplasts are organelles of endosymbiotic origin that perform essential functions in plants. They contain about 3000 different proteins, the vast majority of which are nucleus-encoded, synthesized in precursor form in the cytosol, and transported into the chloroplasts post-translationally. These preproteins are generally imported via envelope complexes termed TOC and TIC (Translocon at the O...
متن کاملMolecular chaperones involved in chloroplast protein import.
Transport of cytoplasmically synthesized precursor proteins into chloroplasts, like the protein transport systems of mitochondria and the endoplasmic reticulum, appears to require the action of molecular chaperones. These molecules are likely to be the sites of the ATP hydrolysis required for precursor proteins to bind to and be translocated across the two membranes of the chloroplast envelope....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1997
ISSN: 1460-2075
DOI: 10.1093/emboj/16.24.7351